Introduction: When blood is stored outside the body, some hematological and biochemical changes take place resulting in reduced red blood cells survival which is an important drawback when transfused into the circulation of a recipient. Objective: The stability of hematological parameters like RBC count, WBC count, differential count, platelet count, MCV, MCH, MCHC and biochemical parameters like S. Sodium, S. Potassium, S. Chloride and albumin during extended storage at 4°C for up to 28 days was evaluated. Materials and Methods: The present research was conducted in L.N. Medical College and J.K. Hospital, Bhopal, in collaboration with blood bank department of our institute. 450 mL of blood was drawn from 30 healthy volunteer donors into citrate phosphate dextrose adenine (CPDA-1) anticoagulant (63 mL). The blood was kept for 28 days and samples were evaluated on days 1, 7, 14, 21 and 28. Results: Among the hematological parameters, there was a constant decline in WBC and platelet counts from day 0 to 28. RBC count, Hb, MCV, HCT showed increasing values; MCH was almost constant, while MCHC decreased. PDW increased while PCT increased till 4th day and then decreased. Neutrophils, Eosinophils, Monocytes decreased, Basophils remained constant while lymphocytes increased. Among the biochemical parameters, values of S. Sodium decreased, S. Chloride decreased till 3rd day, increased on 4th day and then again decreased on 5th day. S. Potassium and albumin showed increasing values.

Introduction: Accurate diagnosis and classification of acute leukemias has helped in identifying and dividing acute leukemias (ALL, AML) into low-risk, intermediate-risk and high-risk groups. Objectives: This study was conducted to determine the clinical profile, hematological profile, immunophenotypic characteristics of acute leukemias in adults, to study correlation between morphological and immunophenotypic characteristics of acute leukemias in adults presenting to a tertiary care center. Material and Methods: 50 subjects who fulfilled the inclusion criteria were enrolled for this cross –sectional study from September 2009 to December 2011.The patients underwent clinical examination, baseline investigations, bone marrow examination and immunophenotypic analysis. Results: The patients of acute leukemias presented with varied symptomatology. The predominant symptoms were moderate- to high-grade fever (94%), bleeding manifestations (52%) [From single site (36%), i.e., epistaxis, hemoptysis, gum bleeding, melena, per vaginal. From multiple sites (16%)], weight loss in (14%), bone and joint pain in (14%), edema in 12%.

The main goal of blood transfusion services (BTS) is provision of safe and adequate supply of blood and blood products. For this, blood for transfusion must be obtained from voluntary non-remunerated blood donors (VNRD).1 Blood transfusion services thus have responsibility of collecting blood only from VNRD who are at low risk for transfusion-transmissible infections (TTIs) and who are unlikely to have other diseases to donate blood1 and at the same time ensure that suitable donors are not unnecessarily deferred.2 The donor selection process requires continuous monitoring to ensure that it achieves its objectives of ensuring donor and recipient safety and providing a sufficient supply of blood and blood components.

Background: In the era of technological advancement, tissue microarray technology (TMA) plays a promising role in supplementing high-throughput molecular analytical studies. Fewer studies in the field of tumor pathology in brain biopsies demand for more analysis using this technique. Aims/ Objectives: The main objective of this study is to validate whether the tumor morphology is conserved in TMA slides in comparison with the conventional H&E slides. Materials and Methods: We analyzed 40 cases of malignant glial tumors (astrocytoma/ oligodendroglioma) using this technology where TMA blocks were constructed using 40 conventionally constructed formalin-fixed paraffin embedded blocks. H&E staining was carried out for all the TMA slides. Results: Out of the 40 cases analyzed, 36 cases (90%) showed positive for tumor. The other 4 cases (10%) showed no evidence of tumor. Out of 36 cases, 31 cases (86.1%) matched the diagnosis done with both the methods. Five cases (13.8%) showed non-concordant diagnosis when compared with the diagnosis given based on conventional H&E slides.

Salivary gland cancers are rare and majority of them occur in the parotid gland. Amongst them, mucoepidermoid carcinoma is the most common malignant salivary gland neoplasm and makes up to 40–50% of malignant parotid gland tumors. They present with diverse clinical presentations. These tumors are typically classified into three histological grades (low, intermediate, or high) which strongly correlate with the clinical behavior and overall prognosis of the patient. The initial diagnosis is based upon the physical examination and the location of the mass within or arising from either major or minor salivary glands. However, in some cases the diagnosis of mucoepidermoid carcinoma may be missed especially where there is an atypical presentation. We herein report one such unusual manifestation of this tumor in a 54- year-old male who was suspected as a case of parotid abscess.

Oral cancer is a subtype of head and neck cancer involving the oral cavity. It ranks among the top three types of cancer in the country and the age-adjusted rate of oral cancer in India is as high as 20 per 100,000 population and accounts for over 30% of all cancers in the country.1 Majority of the oral cancers are squamous cell carcinomas. These arise from the stratified squamous epithelium, which covers the vascularized connective tissue and is keratinized over the hard palate, lips and gingiva while elsewhere it is non-keratinized. A tissue biopsy of the oral lesion is usually obtained and microscopic examination confirms the diagnosis of oral cancer or a precancerous lesion. Globally, as of 2010, 124,000 people have died of oral cancer up from 82,000 in 1990.2 The present study focused on oral squamous cell carcinoma (OSCC) which arises from the stratified squamous epithelium covering the vascularized connective tissue and the common sites encountered where the squamous carcinoma originated were the lateral border of tongue, base of tongue, buccal mucosa, hard/soft palate and tonsils.